Recombinant Human Apolipoprotein A-I 在大腸桿菌 (E. coli) 中表達(dá)。Apolipoprotein A-I (ApoA-I) 是高密度脂蛋白 (HDL) 的主要蛋白成分，在膽固醇反向轉(zhuǎn)運(yùn)途徑中起關(guān)鍵作用。Apolipoprotein A-I (ApoA-I) 與登革熱病毒 (DV) 顆粒相關(guān)，并通過(guò) SR-BI 增強(qiáng)病毒感染。
Synonyms
rHuApolipoprotein A-I; ApoA1; Apolipoprotein A-I
Species
HumanSource
E. coli Accession
P02647 Gene ID
335 Molecular Weight
27-29 kDa AA Sequence
RHFWQQDEPP QSPWDRVKDL ATVYVDVLKD SGRDYVSQFE GSALGKQLNL KLLDNWDSVT STFSKLREQL GPVTQEFWDN LEKETEGLRQ EMSKDLEEVK AKVQPYLDDF QKKWQEEMEL YRQKVEPLRA ELQEGARQKL HELQEKLSPL GEEMRDRARA HVDALRTHLA PYSDELRQRL AARLEALKEN GGARLAEYHA KATEHLSTLS EKAKPALEDL RQGLLPVLES FKVSFLSALE EYTKKLNTQ Biological Activity
Data is not available. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM PB, 150 mM NaCl, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Apolipoprotein A-I (rHuApolipoprotein A-I) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Apolipoprotein A-I (rHuApolipoprotein A-I) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Apolipoprotein A1 (ApoA1) is a 28 kDa glycoprotein that is the major protein component of high density lipoprotein (HDL) particles. HDL particles play a central role in the reverse transport of cholesterol from peripheral tissues to the liver. In the past decade, reconstituted HDL (rHDL) has been employed as a therapeutic agent for treatment of atherosclerosis. The ability of rHDL to promote cholesterol efflux from peripheral cells has been documented to reduce the size of atherosclerotic plaque lesions[2]. |
