Recombinant Human Aldo-keto Reductase 1C4, His 是具有N端His標(biāo)簽的人重組 AKR1C4，來(lái)源于大腸桿菌，對(duì)應(yīng)于人 AKR1C4 的氨基酸 1-323。
Synonyms
rHuAldo-keto Reductase 1C4, His; AKR-1C4; Aldo-keto Reductase 1C4 ; 重組人類醛酮還原酶 1C4，His
Species
HumanSource
E. coli Accession
P17516 Gene ID
1109 Molecular Weight
Approximately 38.0 kDa AA Sequence
HHHHHHMDPK YQRVELNDGH FMPVLGFGTY APPEVPRNRA VEVTKLAIEA GFRHIDSAYL YNNEEQVGLA IRSKIADGSV KREDIFYTSK LWCTFFQPQM VQPALESSLK KLQLDYVDLY LLHFPMALKP GETPLPKDEN GKVIFDTVDL SATWEVMEKC KDAGLAKSIG VSNFNCRQLE MILNKPGLKY KPVCNQVECH PYLNQSKLLD FCKSKDIVLV AHSALGTQRH KLWVDPNSPV LLEDPVLCAL AKKHKQTPAL IALRYQLQRG VVVLAKSYNE QRIRENIQVF EFQLTSEDMK VLDGLNRNYR YVVMDFLMDH PDYPFSDEY Biological Activity
Data is not available. Appearance
Solution Formulation
Supplied as a 0.2 μm filter solution of 20 mM Tris, 150 mM NaCl, pH 8.0. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
N/A Storage & Stability
Recombinant Human Aldo-keto Reductase 1C4, His (rHuAldo-keto Reductase 1C4, His) is stored at -20°C. It is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Shipping with dry ice. Background
Aldo-keto Reductase 1C4 (AKR1C4) is a member of the aldo-keto reductase (AKR) superfamily. AKR1C4 catalyzes the reversible oxidoreduction of various 3α-hydroxysteroids and prostaglandins as well as the oxidation of trans?dihydrodiols of aromatic hydrocarbons and alicyclic alcohols. AKR1C4 acts as NADPH-dependent 3-, 17- and 20-ketosteroid reductases and as 3α-, 17β- and 20α-hydroxysteroid oxidases. AKR1C4 reduces 11-ketodihydrotestosterone (11KDHT) to both 3α- and 3β-hydroxy metabolites. AKR1C4 is important for steroid hormone metabolism primarily through synthesis and clearance of testosterone. AKR1C4 is virtually liver-specific and its high Kcat/Km allows this enzyme to form 5α/5β-tetrahydrosteroids robustly[1][2]. |
