Recombinant Human AG-3, His (HEK293-expressed) 是帶有 N 末端 His 標(biāo)簽的人重組 AG-3，在 HEK293 細(xì)胞中表達(dá)。
Synonyms
rHuAG-3, His; HAG-3; AGR3; AG3 ; 重組人 AG-3，His (HEK293 細(xì)胞表達(dá))
Species
HumanSource
HEK 293 Accession
Q8TD06 Gene ID
155465 Molecular Weight
Approximately 16.0 kDa AA Sequence
IAIKKEKRPP QTLSRGWGDD ITWVQTYEEG LFYAQKSKKP LMVIHHLEDC QYSQALKKVF AQNEEIQEMA QNKFIMLNLM HETTDKNLSP DGQYVPRIMF VDPSLTVRAD IAGRYSNRLY TYEPRDLPLL IENMKKALRL IQSELHHHHH H Biological Activity
Data is not available. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM Tris-HCl, 150 mM NaCl, 2 mM EDTA, pH 8.5. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human AG-3, His (HEK293-expressed) (rHuAG-3, His) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human AG-3, His (HEK293-expressed) (rHuAG-3, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
AG-3 (Anterior gradient 3; AGR3), a member of the protein disulfide isomerase (PDI) family, shares high sequence homology with AG-2. AG-3 is overexpressed in breast, prostate and ovarian cancer. Combined AG-3 and acid mucopolysaccharides could serve as a diagnostic marker for well-differentiated intrahepatic cholangiocarcinoma. AG-3 expression was correlated with the level of differentiation in the serous type of ovarian cancer. AGR3 activates Wnt/β-catenin signalling and promotes the nuclear translocation of β-catenin to upregulate stemness related genes[1]. |
