Recombinant 3C-like Proteinase, His 在大腸桿菌中表達(dá)，N 端帶有一個 His 標(biāo)簽，可介導(dǎo)病毒多聚蛋白的成熟和宿主蛋白的多次切割，以調(diào)節(jié)病毒的翻譯和轉(zhuǎn)錄[1]。
Synonyms
M Proteinase; 3CL Proteinase; 2019-nCoV 3C-like Proteinase
Species
OthersSource
E. coli Accession
YP_009725301.1 Gene ID
N/A Molecular Weight
Approximately 33.0 kDa AA Sequence
HHHHHHSGFR KMAFPSGKVE GCMVQVTCGT TTLNGLWLDD VVYCPRHVIC TSEDMLNPNY EDLLIRKSNH NFLVQAGNVQ LRVIGHSMQN CVLKLKVDTA NPKTPKYKFV RIQPGQTFSV LACYNGSPSG VYQCAMRPNF TIKGSFLNGS CGSVGFNIDY DCVSFCYMHH MELPTGVHAG TDLEGNFYGP FVDRQTAQAA GTDTTITVNV LAWLYAAVIN GDRWFLNRFT TTLNDFNLVA MKYNYEPLTQ DHVDILGPLS AQTGIAVLDM CASLKELLQN GMNGRTILGS ALLEDEFTPF DVVRQCSGVT FQ Biological Activity
Data is not available. Appearance
Solution. Formulation
Supplied as a 0.2 μm filter solution of PBS, 1 mM EDTA, 10% Glycerol, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
N/A Storage & Stability
Recombinant 3C-like Proteinase, His (r3C-like Proteinase, His) is stored at -20°C. It is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Shipping with dry ice. Background
The 3C protease has been regarded as a valid target due to its structural similarity among different picornaviruses and minimal sequence similarity with host proteins; therefore, the development of potent inhibitors against the 3C protease as an antiviral drug is ongoing. Duck hepatitis A virus (DHAV) belongs to the Picornavidea family and is a major threat to the poultry industry[1]. |
