Recombinant Mouse RANK L/TNFSF11 是來(lái)源于 E. coli 的 TNF 相關(guān)的活化誘導(dǎo)細(xì)胞因子。人源和鼠源 RANK L/TNFSF11 的同源性約為 82%。Recombinant Mouse RANK L/TNFSF11 誘導(dǎo) JNK 的激活，增強(qiáng) T 細(xì)胞生長(zhǎng)和樹(shù)突細(xì)胞功能，誘導(dǎo)破骨細(xì)胞生成和淋巴結(jié)器官生成。
Synonyms
rMuRANK L/TNFSF11; TRANCE; CD254
Species
MouseSource
E. coli Accession
O35235 Gene ID
21943 Molecular Weight
Approximately 20.1 kDa AA Sequence
QRFSGAPAMM EGSWLDVAQR GKPEAQPFAH LTINAASIPS GSHKVTLSSW YHDRGWAKIS NMTLSNGKLR VNQDGFYYLY ANICFRHHET SGSVPTDYLQ LMVYVVKTSI KIPSSHNLMK GGSTKNWSGN SEFHFYSINV GGFFKLRAGE EISIQVSNPS LLDPDQDATY FGAFKVQDID Biological Activity
The ED50 is 0.2-1 ng/mL as measured by RAW 264.7 mouse monocyte/macrophage cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM Tris, 150 mM NaCl, 1 mM EDTA, pH 8.0. Endotoxin Level
<1.0 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Mouse RANK L/TNFSF11 (rMuRANK L/TNFSF11) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Mouse RANK L/TNFSF11 (rMuRANK L/TNFSF11) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Receptor activator of NF-κB ligand (RANKL), its cellular receptor, receptor activator of NF-κB (RANK), and the decoy receptor osteoprotegerin (OPG) constitute a novel cytokine system. RANKL produced by osteoblastic lineage cells and activated T lymphocytes is the essential factor for osteoclast formation, fusion, activation, and survival, thus resulting in bone resorption and bone loss. RANKL activates its specific receptor, RANK located on osteoclasts and dendritic cells, and its signaling cascade involves stimulation of the c-jun, NF-κB, and serine/threonine kinase PKB/Akt pathways[1]. |
