Recombinant Mouse Tumor Necrosis Factor alpha, his 是帶有標(biāo)簽的重組小鼠腫瘤壞死因子α (TNF-α)。Recombinant Mouse Tumor Necrosis Factor alpha 是炎癥的主要介質(zhì)，參與了許多傳染病和寄生蟲病。
Synonyms
rMuTNF-α, His; Cachectin; TNFSF2 ; 重組小鼠腫瘤壞死因子α，His 標(biāo)簽
Species
MouseSource
E. coli Accession
P06804 Gene ID
21926 Molecular Weight
Approximately 18.3 kDa AA Sequence
MHHHHHHMLR SSSQNSSDKP VAHVVANHQV EEQLEWLSQR ANALLANGMD LKDNQLVVPA DGLYLVYSQV LFKGQGCPDY VLLTHTVSRF AISYQEKVNL LSAVKSPCPK DTPEGAELKP WYEPIYLGGV FQLEKGDQLS AEVNLPKYLD FAESGQVYFG VIAL Biological Activity
The ED50 is <0.05 ng/mL as measured by L-929 mouse fibrosarcoma cells, corresponding to a specific activity of >2 × 107 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<1.0 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Mouse Tumor Necrosis Factor alpha, His (rMuTNF-α, His) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Mouse Tumor Necrosis Factor alpha, His (rMuTNF-α, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine produced by a wide variety of cell types of mostly hematopoietic, but also of nonhematopoietic, origin. TNFα is instrumental in the immune elimination of various infectious agents such as Candida albicans, Listeria monocytogenes, or mycobacteria and exerts potent proinflammatory effects, e.g. by inducing the expression of adhesion molecules such as VCAM-1, intercellular adhesion molecule 1 (ICAM-1), or E-selectin on endothelial cells and other cell types[1][2]. |
