Recombinant Human Tumor Necrosis Factor alpha, His 是一種帶有標(biāo)簽的人重組腫瘤壞死因子α (TNF-α)。Recombinant Human Tumor Necrosis Factor alpha 是一種多效性細(xì)胞因子，是炎癥的主要介質(zhì)之一，參與多種傳染病和寄生蟲病。似乎與血腦屏障、炎癥、血栓形成和腦損傷相關(guān)的血管變化有關(guān)。
Synonyms
rHuTNF-α, His; Cachectin; TNFSF2 ; 重組人腫瘤壞死因子 α，His 標(biāo)簽
Species
HumanSource
E. coli Accession
P01375 Gene ID
7124 Molecular Weight
Approximately 18.3 kDa AA Sequence
MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL Biological Activity
The ED50 is <0.13 ng/mL as measured by L-929 mouse fibrosarcoma cells, corresponding to a specific activity of >7.69 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<1.0 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Tumor Necrosis Factor alpha, His (rHuTNF-α, His) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Tumor Necrosis Factor alpha, His (rHuTNF-α, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Tumor necrosis factor-α (TNF-α) is a pleiotropic cytokine produced by a wide variety of cell types of mostly hematopoietic, but also of nonhematopoietic, origin. TNFα is instrumental in the immune elimination of various infectious agents such as Candida albicans, Listeria monocytogenes, or mycobacteria and exerts potent proinflammatory effects, e.g. by inducing the expression of adhesion molecules such as VCAM-1, intercellular adhesion molecule 1 (ICAM-1), or E-selectin on endothelial cells and other cell types. Aberrant production of TNFα, however, has been also implicated in the pathogenesis of various diseases, such as rheumatoid arthritis, insulin-dependent diabetes-mellitus, sialoadenitis, and inflammatory bowel disease, in particular Crohn's disease[1][2]. |
