Recombinant Human T Cell Immunoglobulin Mucin 3, Fc Chimera (HEK293-expressed) 是一種屬于T細胞免疫球蛋白樣蛋白 (TIM) 家族的I型跨膜糖蛋白，參與調節(jié)先天免疫和適應性免疫。
Synonyms
rHuTIM-3, Fc Chimera; TIMD-3; CD366; HAVCR2
Species
HumanSource
HEK 293 Accession
Q8TDQ0 Gene ID
84868 Molecular Weight
60-65 kDa AA Sequence
SEVEYRAEVG QNAYLPCFYT PAAPGNLVPV CWGKGACPVF ECGNVVLRTD ERDVNYWTSR YWLNGDFRKG DVSLTIENVT LADSGIYCCR IQIPGIMNDE KFNLKLVIKP AKVTPAPTRQ RDFTAAFPRM LTTRGHGPAE TQTLGSLPDI NLTQISTLAN ELRDSRLAND LRDSGATIR Biological Activity
Binding ability is measured by functional ELISA, 500 ng/mL (100 μL/well) of immoblized recombinant human T Cell Immunoglobulin Mucin 3-Fc Chimera produces 50% optimal binding response approximately 5 ng/mL. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human T Cell Immunoglobulin Mucin 3, Fc Chimera (HEK293-expressed) (rHuTIM-3, Fc Chimera) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human T Cell Immunoglobulin Mucin 3, Fc Chimera (HEK293-expressed) (rHuTIM-3, Fc Chimera) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
T Cell Immunoglobulin Mucin 3 (TIM-3), also known as Hepatitis A virus cellular receptor 2 (HAVCR2), is an immunomodulatory molecule and upregulated in T cells by several cytokines. TIM-3 also influences mast cell function but its transcriptional regulation in mast cells has not been clarified. Human TIM-3 consists of a membrane distal variable immunoglobulin (IgV)-like domain involved in ligand interactions, a membrane proximal mucin domain and a transmembrane domain that is connected to an intracellular cytoplasmic tail involved in phosphotyrosine-dependent signaling[1][2][3]. |
