Recombinant Human TIGIT, Fc Chimera (HEK293-expressed) 是在 HEK293 細(xì)胞中產(chǎn)生的含有352個氨基酸的多肽鏈，帶有C-端的人 IgG1 Fc 片段。T-cell immunoreceptor with Ig and ITIM domains (TIGIT)，也被稱為 V-set 和免疫球蛋白結(jié)構(gòu)域的蛋白9 (VSIG9)、V-set 和跨膜結(jié)構(gòu)域的蛋白3 (VSTM3)。它屬于單通道 I 型膜蛋白，包含免疫球蛋白可變結(jié)構(gòu)域、跨膜結(jié)構(gòu)域和免疫受體酪氨酸抑制基序 (ITIM)。
Synonyms
rHuTIGIT, Fc Chimera; VSIG9; VSTM3; T Cell Immunoreceptor with Ig and ITIM Domains ; 重組人 TIGIT，F(xiàn)C 嵌合體 (HEK293 細(xì)胞表達(dá))
Species
HumanSource
HEK 293 Accession
Q495A1 Gene ID
201633 Molecular Weight
50-55 kDa AA Sequence
MMTGTIETTG NISAEKGGSI ILQCHLSSTT AQVTQVNWEQ QDQLLAICNA DLGWHISPSF KDRVAPGPGL GLTLQSLTVN DTGEYFCIYH TYPDGTYTGR IFLEVLESSV AEHGARFQIP Biological Activity
5 μg/mL (100 μL/well) of immoblized recombinant human CD155-Fc can bind human Biotin-TIGIT-Fc with a linear range of 6.1-48.83 ng/mL. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, 5% trehalose and mannitol. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human TIGIT, Fc Chimera (HEK293-expressed) (rHuTIGIT, Fc Chimera) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human TIGIT, Fc Chimera (HEK293-expressed) (rHuTIGIT, Fc Chimera) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
T cell Ig and ITIM domain (TIGIT) is a transmembrane glycoprotein belonging to a poliovirus receptor family of type 1 proteins that also binds to CD155 and CD112 ligands. TIGIT is expressed on peripheral memory and regulatory CD4+ T cells and NK cells and is upregulated following activation on naive CD4+ T cells. CD155 is a high-affinity receptor for TIGIT expressed on monocytes and CD11c+ human dendritic cells (DCs). TIGIT contains an Ig-like V-type domain and an ITIM in its cytoplasmic domain, suggesting that receptor occupancy may trigger a negative signaling event[1]. |
