Recombinant Mouse Thrombopoietin, His (HEK293-expressed) 是一種在 HEK293 細(xì)胞中產(chǎn)生的帶有標(biāo)簽的重組小鼠血小板生成素 (TPO)，在 HEK293 細(xì)胞中產(chǎn)生。Recombinant Mouse Thrombopoietin 是巨核細(xì)胞生長(zhǎng)和分化的主要調(diào)節(jié)因子。
Synonyms
rMuThpo, His; C-mpl ligand; MGDF ; 重組小鼠血小板生成素，His 標(biāo)簽 (HEK293 細(xì)胞表達(dá))
Species
MouseSource
HEK 293 Accession
P40226 Gene ID
21832 Molecular Weight
62-70 kDa AA Sequence
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKFPNRTSG LLETNFSVTA RTAGPGLLSR LQGFRVKITP GQLNQTSRSP VQISGYLNRT HGPVNGTHGL FAGTSLQTLE ASDISPGAFN KGSLAFNLQG GLPPSPSLAP DGHTPFPPSP ALPTTHGSPP QLHPLFPDPS TTMPNSTAPH PVTMYPHPRN LSQETHHHHH H Biological Activity
The ED50 is <2 ng/mL as measured by MO7e cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Mouse Thrombopoietin, His (HEK293-expressed) (rMuThpo, His) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Mouse Thrombopoietin, His (HEK293-expressed) (rMuThpo, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Thrombopoietin (TPO) is the master cytokine regulator of megakaryopoiesis. In addition to regulation of megakaryocyte and platelet number, TPO is important for maintaining proper hematopoietic stem cell (HSC) function. TPO produced mainly in the liver, stimulates both megakaryocyte colony formation and enhances megakaryocyte maturation in the bone marrow leading to increased platelet production. TPO binds to its receptor, TPOR/MPL, which is expressed on hematopoietic stem cells (HSCs), megakaryocytes and platelets. Circulating TPO concentrations are controlled by MPL via receptor-mediated internalization and degradation, and in part by sensing of senescent platelets by the Ashwell-Morell receptor in the liver[1]. |
