Recombinant Human Surfactant protein-D (HEK293-expressed) 是一種在 HEK293 細(xì)胞中產(chǎn)生的多聚體聚集蛋白，參與先天免疫防御，在肺上皮和非肺上皮細(xì)胞中表達(dá)。Recombinant Human Surfactant protein-D 通過直接與微生物相互作用以及一系列細(xì)胞受體調(diào)節(jié)宿主細(xì)胞反應(yīng)發(fā)揮抗菌作用和抑制炎癥。
Synonyms
rHuSurfactant protein-D; SFTPD; PSP-D; COLEC7; SFTP4 ; 重組人表面活性劑蛋白 D (HEK293 細(xì)胞表達(dá))
Species
HumanSource
HEK 293 Accession
P35247 Gene ID
6441 Molecular Weight
40-45 kDa AA Sequence
AEMKTYSHRT MPSACTLVMC SSVESGLPGR DGRDGREGPR GEKGDPGLPG AAGQAGMPGQ AGPVGPKGDN GSVGEPGPKG DTGPSGPPGP PGVPGPAGRE GPLGKQGNIG PQGKPGPKGE AGPKGEVGAP GMQGSAGARG LAGPKGERGV PGERGVPGNT GAAGSAGAMG PQGSPGARGP PGLKGDKGIP GDKGAKGESG LPDVASLRQQ VEALQGQVQH LQAAFSQYKK VELFPNGQSV GEKIFKTAGF VKPFTEAQLL CTQAGGQLAS PRSAAENAAL QQLVVAKNEA AFLSMTDSKT EGKFTYPTGE SLVYSNWAPG EPNDDGGSED CVEIFTNGKW NDRACGEKRL VVCEF Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Surfactant protein-D (HEK293-expressed) (rHuSurfactant protein-D) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Surfactant protein-D (HEK293-expressed) (rHuSurfactant protein-D) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Surfactant protein-D (SP-D) participates in the innate response to inhaled microorganisms and organic antigens, and contributes to immune and inflammatory regulation within the lung. SP-D is synthesized and secreted by alveolar and bronchiolar epithelial cells, but is also expressed by epithelial cells lining various exocrine ducts and the mucosa of the gastrointestinal and genitourinary tracts. SP-D, a collagenous calcium-dependent lectin (or collectin), binds to surface glycoconjugates expressed by a wide variety of microorganisms, and to oligosaccharides associated with the surface of various complex organic antigens. SP-D also specifically interacts with glycoconjugates and other molecules expressed on the surface of macrophages, neutrophils, and lymphocytes. In addition, SP-D binds to specific surfactant-associated lipids and can influence the organization of lipid mixtures containing phosphatidylinositol in vitro[1][2]. |
