Recombinant Human Sonic Hedgehog, C24II 是一種重組人 sonic hedgehog 的變體。Recombinant Human Sonic Hedgehog 是一種分泌信號轉(zhuǎn)導(dǎo)器，不僅負(fù)責(zé)早期肢體和神經(jīng)發(fā)育過程中前后軸的圖形化，還負(fù)責(zé)在后期發(fā)育階段產(chǎn)生細(xì)胞類型的多樣性。
Synonyms
rHuSonic Hedgehog, C24II; HHG1 ; 重組人 Sonic Hedgehog, C24II
Species
HumanSource
E. coli Accession
Q15465 Gene ID
6469 Molecular Weight
Approximately 19.7 kDa AA Sequence
IIGPGRGFGK RRHPKKLTPL AYKQFIPNVA EKTLGASGRY EGKISRNSER FKELTPNYNP DIIFKDEENT GADRLMTQRC KDKLNALAIS VMNQWPGVKL RVTEGWDEDG HHSEESLHYE GRAVDITTSD RDRSKYGMLA RLAVEAGFDW VYYESKAHIH CSVKAENSVA AKSGG Biological Activity
The ED50 is <2 μg/mL as measured by C3H/10T1/2 (CCL-226) cells, corresponding to a specific activity of >500 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Sonic Hedgehog, C24II (rHuSonic Hedgehog, C24II) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Sonic Hedgehog, C24II (rHuSonic Hedgehog, C24II) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Sonic Hedgehog (SHH) is a secreted factor. The protein is heavily modified through cleavage and posttranslational modification and its release from producing cells can also be modulated by interactions with Dispatched, Scube2, and heparan sulfate proteoglycan[1][2]. |
