Recombinant Human NANOG-TAT是一種多域同源框轉(zhuǎn)錄因子，具有維持多能干細(xì)胞未分化狀態(tài)的功能。
Synonyms
rHuNANOG-TAT; Homeobox protein NANOG ; 重組人NANOG-TAT
Species
HumanSource
E. coli Accession
Q9H9S0 Gene ID
79923 Molecular Weight
Approximately 36.2 kDa AA Sequence
MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA ENSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDVGGYGR KKRRQRRR Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 10 mM PB, 300 mM NaCl, pH 7.4. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human NANOG-TAT (rHuNANOG-TAT) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human NANOG-TAT (rHuNANOG-TAT) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
NANOG is a multidomain homeobox transcription factor which functions to maintain the undifferentiated state of pluripotent stem cells. NANOG expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. Once NANOG expression is downregulated, cell differentiation can proceed. Proteins which regulate NANOG expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53[1]. |
