Recombinant Rat Macrophage Colony Stimulating Factor 是一種促炎性細胞因子，與受體 CSF1R 結(jié)合，參與單核/巨噬細胞的增殖，破骨細胞的誘導(dǎo)，在受損的軟骨及骨質(zhì)疏松等疾病中起到重要作用。
ynonyms
rRtM-CSF; CSF-1; M-CSF ; 重組大鼠巨噬細胞集落刺激因子
Species
RatSource
E. coli Accession
Q6GMN4 Gene ID
78965 Molecular Weight
Approximately 28 kDa AA Sequence
MEVSEHCSHM IGNGHLQILQ QLIDSQMETA CLIEYKFVDQ EQLDDPVCYL KKAFVLVQVI IEETMRFKDN TPNANATERL QELSMKLNSC FIKDYKEQNE ACVQTYKESP LRLLEKIKNF FNETKNFLEK DWNIFSKNCN DSLAKCSSRD VVTKP Biological Activity
The ED50 is <5 ng/mL as measured by murine M-NFS-60 cells, corresponding to a specific activity of >2 × 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50 mM Tris, 150 mM NaCl, pH 8.0. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Macrophage Colony Stimulating Factor (rRtM-CSF) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Rat Macrophage Colony Stimulating Factor (rRtM-CSF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Macrophage Colony Stimulating Factor (M-CSF) is a pro-inflammatory cytokine, constitutively produced by several cell types, such as fibroblasts, endothelial cells, stromal cells, macrophages, smooth muscle cells and osteoblasts, binds to its receptor CSF1R, and exists in several isoforms- as a secreted glycoprotein, a cell-surface protein and a proteoglycan[1]. M-CSF is involved in the development and proliferation of cells of the monocyte/macrophage lineage and participates in the induction of osteoclasts, which are important in the destruction of bone and cartilage and in the periarticular osteoporotic changes seen in patients with rheumatoid arthritis[2]. |
