Recombinant Rat Fractalkine/CX3CL1 (HEK293-expressed) 是 CX3C 趨化因子家族的成員，與多種腫瘤有關(guān)，促進(jìn)腫瘤進(jìn)展。
Synonyms
rRtFractalkine/CX3CL1; Fractalkine; CX3CL1; FKN; SCYD1; Neurotactin; NTT ; 重組大鼠趨化因子/CX3CL1 (HEK293 細(xì)胞表達(dá))
Species
RatSource
HEK 293 Accession
O55145 Gene ID
89808 Molecular Weight
70-90 kDa AA Sequence
QHLGMTKCNI TCHKMTSPIP VTLLIHYQLN QESCGKRAII LETRQHRHFC ADPKEKWVQD AMKHLDHQTA ALTRNGGKFE KRVDNVTPRI TSATRGLSPT ALAKPESATV EDLTLEPTAI SQEARRPMGT SQEPPAAVTG SSPSTSKAQD AGLAAKPQST GISEVAAVST TIWPSSAVYQ SGSSLWAEEK ATESPPTIAL STQASTTSSP KQNVGSEGQP PWVQEQDSTP EKSPGPEETN PVHTDIFQDR GPGSTVHPSV APTSSEKTPS PELVASGSQA PKVEEPIHAT ADPQKLSVFI TPVPDSQAAT Biological Activity
The EC50 is <1 μg/mL as measured on Ca2+ mobilization assay in CHO-K1/Gα15/rCX3CR1 cells (human Gα15 and rat CX3CR1 stably expressed in CHO-K1 cells). Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Fractalkine/CX3CL1 (HEK293-expressed) (rRtFractalkine/CX3CL1) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Rat Fractalkine/CX3CL1 (HEK293-expressed) (rRtFractalkine/CX3CL1) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Fractalkine is the only member of the CX3C chemokine family so far. It is expressed in many cell types such as hematopoietic cells, endothelial cells, epithelial cells, smooth muscle cells, dendritic cells, neurons, and fibroblasts. Fractalkine exists in two forms that have distinct cellular functions. The soluble form is a chemoattractant for cells expressing the CX3CR1 receptor. The membrane-bound form functions as an adhesion molecule that promotes adhesion and migration[1]. |
