Recombinant Human Fibroblast Growth Factor 10, His 是帶有 His 標(biāo)簽的多肽鏈。FGF-10 是結(jié)合肝素的生長因子，屬于 FGF 家族。
Synonyms
rHuFGF-10, His; Fibroblast Growth Factor-10; Keratinocyte growth factor-2
Species
HumanSource
E. coli Accession
O15520 Gene ID
2255 Molecular Weight
Approximately 21.4 kDa AA Sequence
MNHKVHHHHH HMDDDDKMLG QDMVSPEATN SSSSSFSSPS SAGRHVRSYN HLQGDVRWRK LFSFTKYFLK IEKNGKVSGT KKENCPYSIL EITSVEIGVV AVKAINSNYY LAMNKKGKLY GSKEFNNDCK LKERIEENGY NTYASFNWQH NGRQMYVALN GKGAPRRGQK TRRKNTSAHF LPMVVHS Biological Activity
The ED50 is <20 ng/mL as measured by 4MBr-5 cells, corresponding to a specific activity of >5 × 104 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Fibroblast Growth Factor 10, His (rHuFGF-10, His) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Fibroblast Growth Factor 10, His (rHuFGF-10, His) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Fibroblast growth factor 10 (FGF10) regulates multiple stages of structural lung morphogenesis, cellular differentiation, and the response to injury. As a driver of lung airway branching morphogenesis, FGF10 signaling defects during development lead to neonatal lung disease. Alternatively, congenital airway abnormalities attributed to FGF10 mutations increase the risk of chronic airway disease in adulthood. FGF10 also maintains progenitor cell populations in the airway and promotes alveolar type 2 cell expansion and differentiation following injury. Here we review the cellular and molecular mechanisms linking FGF10 to multiple lung diseases, from bronchopulmonary dysplasia in extremely preterm neonates, cystic fibrosis in children, and chronic adult lung disorders. Understanding the connections between FGF10 and lung diseases may lead to exciting new therapeutic strategies[1]. |
