Recombinant Mouse Betacellulin 是 E. coli 產(chǎn)生的表皮生長(zhǎng)因子家族的一員，可誘導(dǎo)胰腺 β 細(xì)胞的分化，促進(jìn) β 細(xì)胞的再生。
Synonyms
rMuBetacellulin; BTC
Species
MouseSource
E. coli Accession
Q05928 Gene ID
12223 Molecular Weight
Approximately 9.2 kDa AA Sequence
MDGNTTRTPE TNGSLCGAPG ENCTGTTPRQ KVKTHFSRCP KQYKHYCIHG RCRFVVDEQT PSCICEKGYF GARCERVDLF Y Biological Activity
The ED50 is <0.5 ng/mL as measured by 3T3 cells, corresponding to a specific activity of >2 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50 mM Tris, 300 mM NaCl, pH 9.0. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Mouse Betacellulin (rMuBetacellulin) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Mouse Betacellulin (rMuBetacellulin) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Betacellulin (BTC), a member of the epidermal growth factor (EGF) family, induces differentiation of pancreatic β-cells and promotes regeneration of β-cells in experimental diabetes. BTC stimulates DNA synthesis in fibroblasts and vascular smooth muscle cells. BTC plays a role in regulating growth and/or differentiation of endocrine precursor cells of the fetal pancreas. BTC is found to convert amylase-secreting pancreatic AR42J cells into insulin-producing cells and to have a mitogenic effect in human undifferentiated pancreatic epithelial cells[1]. |
