Recombinant Human B7-H3/ICOSLG, Fc Chimera (HEK293-expressed) is a polypeptide chain containing the C-termimal human IgG1 Fc fragment produced in HEK293 cells. B7-H3 is an immune checkpoint from the B7 family of molecules. Recombinant Human B7-H3/ICOSLG, Fc Chimera (HEK293-expressed) 是 HEK293 細(xì)胞中產(chǎn)生的 C-末端含人 IgG1 Fc 片段的多肽鏈。B7-H3 是來自 B7 分子家族的免疫檢查點(diǎn)。
Synonyms
rHuB7-H3/ICOSLG, Fc Chimera; B7H3; B7 homolog 3; CD276
Species
HumanSource
HEK 293 Accession
Q5ZPR3 Gene ID
80381 Molecular Weight
62-65 kDa AA Sequence
LEVQVPEDPV VALVGTDATL CCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FAEGQDQGSA YANRTALFPD LLAQGNASLR LQRVRVADEG SFTCFVSIRD FGSAAVSLQV AAPYSKPSMT LEPNKDLRPG DTVTITCSSY RGYPEAEVFW QDGQGVPLTG NVTTSQMANE QGLFDVHSVL RVVLGANGTY SCLVRNPVLQ QDAHGSVTIT GQPMTFP Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human B7-H3/ICOSLG, Fc Chimera (HEK293-expressed) (rHuB7-H3/ICOSLG, Fc Chimera) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human B7-H3/ICOSLG, Fc Chimera (HEK293-expressed) (rHuB7-H3/ICOSLG, Fc Chimera) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
As a member of the B7 family, inducible co-stimulator ligand (ICOSLG) expressed on tumor cell has been reported to have an important role in tumor immunity. As the counter ligand of ICOS, a CD28-related molecule, ICOSLG is expressed on professional antigen-presenting cell (APCs; such as dendritic cells (DCs) and B cells) and on some tumor cells (such as glioma cells and gastric carcinoma cells). ICOSLG is the only B7 family member that preferentially co-stimulates type 2T helper cell (Th2) responses, and interestingly, this unique molecule has a critical role in antitumor immunity. ICOSLG expression on solid tumors (implanted-transfected tumors) aids in both NK mediated and CD8+ cytotoxic T cell (CTL) activation and killing. Several studies using ICOSLG-transfected solid tumor cell lines found that ICOSLG induced CD8+ cytotoxic lymphocyte-mediated tumor regression[1]. |
