Recombinant Rat VEGF164 (P. pastoris-expressed) 是一種有效的血管生成的調(diào)節(jié)劑。
Synonyms
rRtVEGF164; VEGF-A; VPF ; 重組大鼠血管內(nèi)皮生長因子164 (P. pastoris 表達)
Species
RatSource
P. pastoris Accession
QB5DEK7 Gene ID
83785 Molecular Weight
Approximately 38 kDa AA Sequence
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPENHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR Biological Activity
The ED50 is <4 ng/mL as measured by cell proliferation assay of HUVEC. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat VEGF164 (P.pastoris-expressed) (rRtVEGF164) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Rat VEGF164 (P.pastoris-expressed) (rRtVEGF164) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Vascular endothelial growth factor (VEGF or VEGFA), is a potent mediator of angiogenesis in the fetus and adult. Rat VEGF164 shares 97% aa sequence identity with corresponding regions of mouse, 88% with human and bovine, 89% with porcine and canine, and 90% with feline and equine VEGF, respectively. Human VEGF165 binds the semaphorin receptor, neuropilin1 and promotes complex formation with VEGF R2. VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells[1][2]. |
