Recombinant Human Tumor Necrosis Factor beta 具有抗增殖活性，破壞腫瘤細(xì)胞系，能在免疫調(diào)節(jié)中發(fā)揮多種重要作用。
Synonyms
rHuTNF-β; TNF-β; TNFSF1; Lymphotoxin-alpha ; 重組人腫瘤壞死因子 β
Species
HumanSource
E. coli Accession
P01374 Gene ID
4049 Molecular Weight
Approximately 18.6 kDa AA Sequence
MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL Biological Activity
The ED50 is <4 pg/mL as measured by L-929 mouse fibrosarcoma cells, corresponding to a specific activity of >2.5 × 108 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Reconstitution
Reconstitute the lyophilized recombinant Human Tumor Necrosis Factor beta (rHuTNF-β) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Human Tumor Necrosis Factor beta (rHuTNF-β) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
As a signaling molecule, LT-α (TNF-β, Tumor Necrosis Factor beta) is involved in the regulation of cell survival, proliferation, differentiation, and apoptosis[1]. TNF-β plays an important role in innate immune regulation and its presence has been shown to prevent tumor growth and destroy cancerous cell lines[2]. In contrast, unregulated expression of TNF-β can result in a constantly active signaling pathway, thus leading to uncontrolled cellular growth and creation of tumors[1]. Hence depending on the context, TNF-β may function to prevent growth of cancer cells or facilitate the development of tumors. Furthermore, TNF-β effects depend on the type of organ it acts upon, type of cancer cells, cellular environment, gender, and time of effect during an immune response[2]. |
