Recombinant Bovine Tumor Necrosis Factor alpha 是一種參與全身炎癥反應的細胞因子，是構成急性時相反應的細胞因子之一。
Synonyms
rBoTNF-α; Cachectin; TNFA; TNF-α ; 重組牛腫瘤壞死因子α
Species
BovineSource
E. coli Accession
Q06599 Gene ID
280943 Molecular Weight
Approximately 17.6 kDa AA Sequence
MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL Biological Activity
The ED50 is <0.1 μg/mL as measured by mouse L-929 cells, corresponding to a specific activity of >1.0 × 104 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Bovine Tumor Necrosis Factor alpha (rBoTNF-α) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Bovine Tumor Necrosis Factor alpha (rBoTNF-α) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Tumor Necrosis Factor alpha is a cytokine involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. Bovine tumor necrosis factor-α alters luteal cell synthetic capacity and viability[1]. |
