Recombinant Human TECK/CCL25 參與T細胞的發(fā)育和細胞遷移小腸的過程。
Synonyms
rHuTECK/CCL25; C-C motif chemokine 25; SCYA25 ; 重組人 CC 趨化因子25
Species
HumanSource
E. coli Accession
O15444 Gene ID
6370 Molecular Weight
Approximately 14.3 kDa AA Sequence
MQGVFEDCCL AYHYPIGWAV LRRAWTYRIQ EVSGSCNLPA AIFYLPKRHR KVCGNPKSRE VQRAMKLLDA RNKVFAKLHH NTQTFQAGPH AVKKLSSGNS KLSSSKFSNP ISSSKRNVSL LISANSGL Biological Activity
Full biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 150 mM NaCl. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human TECK/CCL25 (rHuTECK/CCL25) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA. Storage & Stability
Lyophilized recombinant Human TECK/CCL25 (rHuTECK/CCL25) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Thymus-expressed chemokine (TECK) or CCL25, a CC chemokine expressed predominantly in thymus and epithelium of the small intestine and a ligand of CCR9, mediates chemotaxis of CCR9-bearing T cells. CCL25 is the predominant chemokine involved in cellular migration to the small intestine[1]. CCL25 mediates the localization of recently activated CD8αβ+ lymphocytes to the small-intestinal mucosa[2]. |
