Recombinant Mouse Sonic Hedgehog 是一種能夠協(xié)調(diào)多種小腦發(fā)育成熟過程的形態(tài)發(fā)生因子。
Synonyms
rMuShh; HHG-1; ShhNC ; 重組鼠源音猬因子
Species
MouseSource
CHO Accession
Q62226 Gene ID
20423 Molecular Weight
Approximately 20 kDa AA Sequence
CGPGRGFGKR RHPKKLTPLA YKQFIPNVAE KTLGASGRYE GKITRNSERF KELTPNYNPD IIFKDEENTG ADRLMTQRCK DKLNALAISV MNQWPGVKLR VTEGWDEDGH HSEESLHYEG RAVDITTSDR DRSKYGMLAR LAVEAGFDWV YYESKAHIHC SVKAENSVAA KSGG Biological Activity
The ED50 is <1 μg/mL as measured by CCL-226 cells, corresponding to a specific activity of >1.0 × 103 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Mouse Sonic Hedgehog (CHO-expressed) (rMuShh) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Mouse Sonic Hedgehog (CHO-expressed) (rMuShh) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Sonic hedgehog (Shh) is a morphogenic factor that actively orchestrates many aspects of cerebellar development and maturation[1]. Sonic hedgehog (Shh) plays a critical role in post-natal skeletal muscle regeneration. Sonic hedgehog (Shh) is a crucial morphogen that regulates epithelial-mesenchymal interactions during embryogenesis. In adults, the Shh pathway has been shown to be up-regulated following skeletal muscle and myocardium ischemia, suggesting that the embryonic Shh pathway can be recruited[2]. |
