Recombinant Rat Platelet-Derived Growth Factor-BB 屬于 PDGF 家族，通過(guò)與 PDGF 受體結(jié)合，促進(jìn)細(xì)胞增殖、存活和遷移。
Synonyms
rRtPDGF-BB; PDGF-2; Pdgfb ; 重組大鼠血小板衍生生長(zhǎng)因子-BB
Species
RatSource
E. coli Accession
Q05028 Gene ID
24628 Molecular Weight
Approximately 24.6 kDa AA Sequence
MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPVFKKATV TLEDHLACKC ETVVTPRPVT Biological Activity
The ED50 is <2 ng/mL as measured by 3T3 cells, corresponding to a specific activity of >5.0 × 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM acetic acid. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Platelet-Derived Growth Factor-BB (rRtPDGF-BB) to 100 μg/mL using 20 mM acetic acid. Storage & Stability
Lyophilized recombinant Rat Platelet-Derived Growth Factor-BB (rRtPDGF-BB) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Platelet derived growth factor (PDGF) is a potent mitogen and chemoattractant for mesenchymal and osteogenic cells and stimulates angiogenic molecules which play an essential role in bone regeneration[1]. Platelet-Derived Growth Factor-BB is a member of PDGF family, which promotes cell proliferation, survival and migration, through binding to the tyrosine kinase PDGF receptor. PDGF-BB exerts beneficial effects on haemorrhagic shock, which are closely related to targeting CX43 to improve vascular reactivity and haemodynamics. PDGF-BB functions in corporal cavernosum smooth muscle cells (CCSMCs) via binding to PDGFR[2]. |
