Recombinant Human MIP-1 alpha/CCL3 (CHO-expressed) 是 CC 亞家族成員，能夠與三種細(xì)胞表面受體 (CCR1，CCR3 和 CCR5) 結(jié)合，具有多種生物活性。
Synonyms
rHuMIP-1α/CCL3; C-C motif chemokine 3; MIP1A; SCYA3 ; 重組人 MIP-1 alpha/CCL3 (CHO 細(xì)胞表達(dá))
Species
HumanSource
CHO Accession
P10147 Gene ID
6348 Molecular Weight
8-10 kDa AA Sequence
ADTPTACCFS YTSRQIPQNF IADYFETSSQ CSKPGVIFLT KRSRQVCADP SEEWVQKYVS DLELSA Biological Activity
The ED50 is <100 ng/mL as measured by CHO/Gα15 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human MIP-1 alpha/CCL3 (CHO-expressed) (rHuMIP-1α/CCL3) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Human MIP-1 alpha/CCL3 (CHO-expressed) (rHuMIP-1α/CCL3) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
CCL3 also known as macrophage inflammatory protein 1-a, is a member of the CC subfamily. It’s known that CCL3 is produced by monocytes/macrophages, lymphocytes, neutrophils as well as immune cells such as basophils, mast cells, fibroblasts, and dendritic cells. Meanwhile, CCL3 exerts various biological effects by binding to its three cell surface receptors, including CCR1, CCR3, and CCR5. MIP-1a induces a variety of pro-inflammatory activities such as leukocyte chemotaxis, and promotes the entry of T cells into the inflammatory tissue region from blood circulation. Chemotactic CD4+ cells, CD8+ cells, natural killer cells, and dendritic cells bind to the corresponding receptors and coordinate the occurrence of immune reactions in the immune response site by migrating through vascular endothelial cells. In addition, MIP-1a is considered as a key inflammatory mediator in granuloma, asthma, T1D as well as other autoimmune diseases[1]. |
