Recombinant Human Monocyte Chemotactic Protein-1/CCL2 是屬于CC趨化因子家族的細(xì)胞因子。
Synonyms
rHuMCP-1/CCL2; C-C motif chemokine 2; MCAF; MCP-1; SCYA2 ; 重組人單核細(xì)胞趨化蛋白1
Species
HumanSource
E. coli Accession
P13500 Gene ID
6347 Molecular Weight
Approximately 8.7 kDa AA Sequence
QPDAINAPVT CCYNFTNRKISVQR LASYRRITSS KCPKEAVIFK TIVAKEICAD PKQKWVQDSM DHLDKQTQTP KT Biological Activity
The ED50 is <1 μg/mL as measured by CHO-K1/Gα15/hCCR2 cells (human Gα15 and human CCR2 stably expressed in CHO-K1 cells). Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Monocyte Chemotactic Protein-1/CCL2 (rHuMCP-1/CCL2) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Human Monocyte Chemotactic Protein-1/CCL2 (rHuMCP-1/CCL2) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
CCL2 is produced by a variety of cell types, either constitutively or after induction by oxidative stress, cytokines, or growth factors. CCL2 is produced by many cell types, including endothelial, fibroblasts, epithelial, smooth muscle, mesangial, astrocytic, monocytic, and microglial cells. CCL2 regulates the migration and infiltration of monocytes, memory T lymphocytes, and natural killer (NK) cells[1]. The chemokine CCL2 and its main chemokine receptor CCR2 have been implicated in the pathogenesis of several different disease processes, including vascular permeability and attraction of immune cells during metastasis, a number of different neurological disorders, autoimmune disease, obesity, and atherosclerosis[2]. |
