Recombinant Human Leptin是脂肪細(xì)胞分泌的一種激素，通過(guò)抑制攝食和增加產(chǎn)熱來(lái)幫助調(diào)節(jié)能量平衡。
Synonyms
rHuLeptin; Obesity protein; OB ; 重組人瘦素
Species
HumanSource
E. coli Accession
P41159 Gene ID
3952 Molecular Weight
Approximately 16.0 kDa AA Sequence
VPIQKVQDDT KTLIKTIVTR INDISHTQSV SSKQKVTGLD FIPGLHPILT LSKMDQTLAV YQQILTSMPS RNVIQISNDL ENLRDLLHVL AFSKSCHLPW ASGLETLDSL GGVLEASGYS TEVVALSRLQ GSLQDMLWQL DLSPGC Biological Activity
The ED50 is <2 ng/mL as measured by human Leptin R transfected BaF3 murine proB cells, corresponding to a specific activity of >5.0 × 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Leptin (rHuLeptin) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA. Storage & Stability
Lyophilized recombinant Human Leptin (rHuLeptin) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
A sensor (leptin production by adipose cells) monitors the size of the adipose tissue mass. Hypothalamic centers receive and integrate the intensity of the leptin signal through leptin receptors (LRb). Effector systems, including the sympathetic nervous system, control the two main determinants of energy balance-energy intake and energy expenditure[1]. Recessive mutations in the leptingene are associated with massive obesity in mice and humans, establishing a genetic basis for obesity. Leptin circulates in blood and acts on the brain to regulate food intake and energy expenditure. When fat mass falls, plasma leptin levels fall, stimulating appetite and suppressing energy expenditure until fat mass is restored. When fat mass increases, leptin levels increase, suppressing appetite until weight is lost. This system maintains homeostatic control of adipose tissue mass[2]. |
