Recombinant Canine Interleukin-3是一種造血生長(zhǎng)因子，參與多能造血細(xì)胞的存活，增殖和分化。
Synonyms
rCaIL-3; Mast cell growth factor; MCGF; Hematopoietic growth factor ; 重組犬白細(xì)胞介素-3
Species
CanineSource
E. coli Accession
Q9BDX4 Gene ID
481497 Molecular Weight
Approximately 14.0 kDa AA Sequence
RPFSTDLPKQ YFTMINEIME MLNKSPSPSE EPLDSNEKET LLEDTLLRPN LDVFLNASSK FHKNGLLIWN NLKEFLPLLP TPTPRGEPIS IMENNWGDFQ RKLKKYLEAL DNFLNFKNKP Biological Activity
The ED50 is <0.2 ng/mL as measured by human TF-1 cells, corresponding to a specific activity of >5.0 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Canine Interleukin-3 (rCaIL-3) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA. Storage & Stability
Lyophilized recombinant Canine Interleukin-3 (rCaIL-3) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin-3 (IL-3) is a multipotent hematopoietic growth factor produced by activated T cells, monocytes/macrophages and stroma cells. Addition of IL-3 to the culture medium induces proliferation, maturation and probably self-renewal of pluripotent hematopoietic stem cells and cells of myeloid, erythroid and megakaryocytic lineages[1]. IL-3 preferentially supports the proliferation and differentiation of progenitors at early stages of hematopoietic development. In addition, IL-3 exerts a wide spectrum of biological activities on various target cell populations, including T cells, B cells, eosinophils, basophils and monocytes[2]. |
