Recombinant Human Interleukin-20是IL-10細(xì)胞因子家族的成員。 Recombinant Human Interleukin-20與IL-20異二聚體受體結(jié)合，Kd 值為 1.5 nM。
Synonyms
rHuIL-20; IL20; Cytokine Zcyto10 ; 重組人白細(xì)胞介素-20
Species
HumanSource
E. coli Accession
Q9NYY1 Gene ID
50604 Molecular Weight
Approximately 17.6 kDa AA Sequence
MLKTLNLGSC VIATNLQEIR NGFSEIRGSV QAKDGNIDIR ILRRTESLQD TKPANRCCLL RHLLRLYLDR VFKNYQTPDH YTLRKISSLA NSFLTIKKDL RLCHAHMTCH CGEEAMKKYS QILSHFEKLE PQAAVVKALG ELDILLQWME ETE Biological Activity
The ED50 is <0.5 ng/mL as measured by murine BaF3 pro-B cells, corresponding to a specific activity of >2.0 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.2, with trehalose. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Interleukin-20 (rHuIL-20) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1% BSA. Storage & Stability
Lyophilized recombinant Human Interleukin-20 (rHuIL-20) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Overexpression of Interleukin-20 (IL-20) in transgenic (TG) mice causes neonatal lethality with skin abnormalities including aberrant epidermal differentiation. Recombinant IL-20 protein stimulates a signal transduction pathway through STAT3 in a keratinocyte cell line, demonstrating a direct action of this ligand. An IL-20 receptor is identified as a heterodimer of two orphan class II cytokine receptor subunits[1]. |
