Recombinant Porcine Interleukin-1 beta 能調(diào)控炎癥反應(yīng)，參與細(xì)胞增殖、分化、凋亡等多種細(xì)胞生命活動(dòng)。
Synonyms
rPoIL-1β; IL1B ; 重組豬白細(xì)胞介素-1 β
Species
PorcineSource
E. coli Accession
P26889 Gene ID
397122 Molecular Weight
Approximately 17.7 kDa AA Sequence
MANVQSMECK LQDKDHKSLV LAGPHMLKAL HLLTGDLKRE VVFCMSFVQG DDSNNKIPVT LGIKGKNLYL SCVMKDNTPT LQLEDIDPKR YPKRDMEKRF VFYKTEIKNR VEFESALYPN WYISTSQAEQ KPVFLGNSKG RQDITDFTME VLSP Biological Activity
The ED50 is <1 ng/mL as measured by mouse D10S cells, corresponding to a specific activity of >1.0 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Porcine Interleukin-1 beta (rPoIL-1β) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Porcine Interleukin-1 beta (rPoIL-1β) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin-1 beta (IL-1 β) is one of the major inflammatory cytokines found in atherosclerotic lesions[1]. Interleukin-1β (IL-1β) acts throughout the IL-1β system, which contains IL-1β and the IL-1β receptor (IL-1R), accessory protein (IL-1RacP), and receptor antagonist (IL-1Ra). In pigs, the expression of the members of the IL-1β system was documented in uterine tissues during the oestrous cycle and early pregnancy, as well as in embryos harvested during the peri-implantation period[2]. Interleukin-1 beta regulates cell proliferation and activity of extracellular matrix remodelling enzymes in cultured primary pig heart cells[3]. |
