| Recombinant Mouse Fibroblast Growth Factor-18 是成熟軟骨細(xì)胞及其祖細(xì)胞的營養(yǎng)因子。 Synonyms
rMuFGF-18; zFGF5; Fgf18 ; 重組小鼠成纖維細(xì)胞生長因子-18
Species
Mouse Source
E. coli Accession
O89101 Gene ID
14172 Molecular Weight
Approximately 20.1 kDa AA Sequence
EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQAELQK PFKYTTVTKR SR Biological Activity
The ED50 is <0.5 μg/mL as measured by 3T3 cells, corresponding to a specific activity of >2.0 × 103 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Mouse Fibroblast Growth Factor-18 (rMuFGF-18) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Mouse Fibroblast Growth Factor-18 (rMuFGF-18) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Mouse Fibroblast Growth Factor-18 (FGF18) is a trophic factor for mature chondrocytes and their progenitors[1]. FGF18 has been reported to have significant anabolic effects on cartilage. FGF18 plays a central role in skeletal growth and development. Mice lacking Fgf18 exhibit malformations in cartilage and bone, including delayed closure of the calvarial sutures, enlargement of the proliferating and hypertrophic zones in the growth plate of long bones, defects in joint development, and delays in osteogenic differentiation[2]. |
