Recombinant Mouse DKK-1 (CHO-expressed) 是 Wnt信號(hào)通路的有效抑制劑，是dickkopf 家族成員之一。
Synonyms
rMuDKK-1; mDkk-1; Dickkopf-1 ; 重組小鼠 DKK-1 (CHO細(xì)胞表達(dá))
Species
MouseSource
CHO Accession
O54908 Gene ID
13380 Molecular Weight
19-20 kDa AA Sequence
SATLNSVLIN SNAIKNLPPP LGGAGGQPGS AVSVAPGVLY EGGNKYQTLD NYQPYPCAED EECGSDEYCS SPSRGAAGVG GVQICLACRK RRKRCMRHAM CCPGNYCKNG ICMPSDHSHF PRGEIEESII ENLGNDHNAA AGDGYPRRTT LTSKIYHTKG QEGSVCLRSS DCAAGLCCAR HFWSKICKPV LKEGQVCTKH KRKGSHGLEI FQRCYCGEGL ACRIQKDHHQ AS Biological Activity
The ED50 is <6 μg/mL as measured in stimulation of alkaline phosphatase activity using CCl-226 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Mouse DKK-1 (CHO-expressed) (rMuDKK-1) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Mouse DKK-1 (CHO-expressed) (rMuDKK-1) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Mature mouse Dkk-1 is a 40 kDa glycosylated protein that shares 86%, 96%, 83% and 82% amino acid (aa) sequence identity with human, rat, rabbit and bovine Dkk-1, respectively. It also shares 41% and 36% aa identity with human Dkk-2 and Dkk-4, respectively[1]. Dkk1 is a secreted Wnt inhibitor and member of a distinct multigene family, this inhibition plays a key role in heart, head and forelimb development during anterior morphogenesis of the embryo[2][3]. |
