Recombinant Human Ciliary Neurotrophic Factor 是一種多能性神經(jīng)營養(yǎng)因子，具有肌營養(yǎng)活性，也被認(rèn)為是具有調(diào)節(jié)成骨細(xì)胞功能的肌動蛋白。
Synonyms
rHuCNTF; Ciliary Neurotrophic Factor ; 重組人睫狀神經(jīng)營養(yǎng)因子 (CNTF)
Species
HumanSource
E. coli Accession
P26441 Gene ID
1270 Molecular Weight
Approximately 22.8 kDa AA Sequence
AFTEHSPLTP HRRDLCSRSI WLARKIRSDL TALTESYVKH QGLNKNINLD SADGMPVAST DQWSELTEAE RLQENLQAYR TFHVLLARLL EDQQVHFTPT EGDFHQAIHT LLLQVAAFAY QIEELMILLE YKIPRNEADG MPINVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRFISS HQTGIPARGS HYIANNKKM Biological Activity
The ED50 is <200 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >5 × 103 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Ciliary Neurotrophic Factor (rHuCNTF) to 100 μg/mL using ddH2O or PBS. Storage & Stability
Lyophilized recombinant Human Ciliary Neurotrophic Factor (rHuCNTF) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Ciliary Neurotrophic Factor (CNTF) has neuroprotective effects on a variety of central and also peripheral nervous system neurons. Because it promotes differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes under in vitro conditions and thus improves remyelination. Importantly, it also increases the survival of mature oligodendrocytes[1]. CNTF has potent effects on the development and maintenance of the nervous system, inducing neuronal survival and differentiation by stimulating gene expression of sensory, sympathetic and motor neurons[2]. |
