Recombinant Human Hepatocyte Growth Factor (CHO-expressed) 是一種細(xì)胞因子，能夠與 Met 激酶受體結(jié)合，具有多種生物活性。
Synonyms
rHuHGF; Hepatopoietin-A; SF ; 重組人肝細(xì)胞生長因子 (CHO 細(xì)胞表達(dá))
Species
HumanSource
CHO Accession
P14210 Gene ID
3082 Molecular Weight
88-90 kDa AA Sequence
QRKRRNTIHE FKKSAKTTLI KIDPALKIKT KKVNTADQCA NRCTRNKGLP FTCKAFVFDK ARKQCLWFPF NSMSSGVKKE FGHEFDLYEN KDYIRNCIIG KGRSYKGTVS ITKSGIKCQP WSSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG LMDHTESGKI CQRWDHQTPH RHKFLPERYP DKGFDDNYCR NPDGQPRPWC YTLDPHTRWE YCAIKTCADN TMNDTDVPLE TTECIQGQGE GYRGTVNTIW NGIPCQRWDS QYPHEHDMTP ENFKCKDLRE NYCRNPDGSE SPWCFTTDPN IRVGYCSQIP NCDMSHGQDC YRGNGKNYMG NLSQTRSGLT CSMWDKNMED LHRHIFWEPD ASKLNENYCR NPDDDAHGPW CYTGNPLIPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLRVVNGIPT RTNIGWMVSL RYRNKHICGG SLIKESWVLT ARQCFPSRDL KDYEAWLGIH DVHGRGDEKC KQVLNVSQLV YGPEGSDLVL MKLARPAVLD DFVSTIDLPN YGCTIPEKTS CSVYGWGYTG LINYDGLLRV AHLYIMGNEK CSQHHRGKVT LNESEICAGA EKIGSGPCEG DYGGPLVCEQ HKMRMVLGVI VPGRGCAIPN RPGIFVRVAY YAKWIHKIIL TYKVPQS Biological Activity
The ED50 is <10 ng/mL as measured by 4MBr5 cells, corresponding to a specific activity of >1 × 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Hepatocyte Growth Factor (CHO-expressed) (rHuHGF) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Hepatocyte Growth Factor (CHO-expressed) (rHuHGF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Hepatocyte growth factor (HGF) exhibits biological activities through the Met tyrosine kinase receptor. HGF plays a role in the regeneration and protection from injury and pathology in various tissues. Genetic disruption of Met in renal cells demonstrates that the HGF-Met pathway plays a role in the proliferation, morphogenesis, and survival of renal cells[1]. |
