TGFβ1是一種多功能細(xì)胞因子，參與胎兒發(fā)育，控制細(xì)胞生長和分化，誘導(dǎo)纖維化，引起免疫應(yīng)答的抑制，參與血管生成，腫瘤的發(fā)展和炎癥過程。
Synonyms
TGF-beta-1; TGFB1; TGFB; rHuTGF-β1
Species
HumanSource
CHO Accession
P01137 Gene ID
7040 Molecular Weight
Approximately 12 kDa AA Sequence
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS Biological Activity
The ED50 is <0.2 ng/mL as measured by HT-2 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50 mM NaAc, 50 mM NaCl, pH 5.0. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Transforming Growth Factor β1 (rHuTGF-β1) to 100 μg/mL using ddH2O or diluted with 50 mM Citrate. Storage & Stability
Lyophilized recombinant Human Transforming Growth Factor β1 (rHuTGF-β1) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
TGFβ is released from degranulating platelets and secreted by all of the major cell types participating in the repair process, including lymphocytes, macrophages, endothelial cells, smooth muscle cells, epithelial cells, and fibroblasts. Mammals express three isoforms of TGFβdesignated TGFβI, TGFβ2, and TGFβ3; TGFβ1 is the most abundant isoform in all tissues, and in human platelets it is the only isoform of the peptide. Certain cells such as retinal pigment epithelial cells secrete predominantly TGFβ2, and certain body fluids such as the aqueous and vitreous of the eye, amniotic fluid, saliva, and breast milk contain principally TGFβ2. TGFβ3 is the least studied of the TGFβ isoforms. It has been isolated from human umbilical cord and is secreted from certain cells, including myoblast celllines; however, it is usually less abundant than either TGFβ1 or TGFβ2 in both tissue and cell extracts[1]. There are three fundamental directions of its activities: I. TGFβ1 regulates cell proliferation, growth, differentiation and cells movement. II. TGFβ1 has immunomodulatory effects. III. TGFβ1 has profibrogenic effects. TGFβ1 action can be local and systemic[2]. |
