Recombinant Human R-spondin-1/RSPO1是一種激活Wnt信號傳導(dǎo)的分泌蛋白。
Synonyms
rHuR-spondin-1/RSPO1; Roof plate-specific spondin-1
Species
HumanSource
CHO Accession
Q2MKA7 Gene ID
284654 Molecular Weight
Approximately 25.6 kDa AA Sequence
RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPAHHHHHH Biological Activity
The ED50 is <0.1 ug/ml measured by its ability to bind Mouse CD36 in functional ELISA. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human R-spondin-1/RSPO1 (rHuR-spondin-1/RSPO1) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human R-spondin-1/RSPO1 (rHuR-spondin-1/RSPO1) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
The R-Spondin (RSpo) family of secreted proteins act as potent activators of the Wnt/ -catenin signaling pathway. R-spondin-1/RSPO1 activity critically depends on the presence of canonical Wnt ligands and LRP6[1]. Although R-spondin-1/RSPO1 does not directly activate LRP6, R-spondin-1/RSPO1 can interfere with DKK1 function, which is to regulate the turnover of the LRP5 or LRP6 receptor and to amplify Wnt-dependent signaling[2]. |
