Recombinant Human 4-1BB Ligand/TNFSF9是TNF家族的共刺激成員，在活化的B細(xì)胞，巨噬細(xì)胞和樹突細(xì)胞上表達(dá)。
Synonyms
rHu4-1BBL/TNFSF9; CD137L
Species
HumanSource
E. coli Accession
P41273 Gene ID
8744 Molecular Weight
Approximately 19.3 kDa AA Sequence
REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM PBS, 150 mM NaCl, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human 4-1BB Ligand/TNFSF9 (rHu4-1BBL/TNFSF9) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human 4-1BB Ligand/TNFSF9 (rHu4-1BBL/TNFSF9) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
4-1BB Ligand/TNFSF9 is expressed on activated B cells, macrophages, and dendritic cells. 4-1BB Ligand-expressing cell lines or soluble 4-1BB Ligand induce mitogen-activated, CD3-cross-linked, or alloreactive T cells to proliferate and secrete IL-2 in vitro. 4-1BB Ligand is important for the generation of antiviral CD8 T cell responses[1]. 4-1BB Ligand enhances proliferation, cytokine production, and CTL effector function of TCR transgenic T cells. Both CD4 and CD8 T cells respond to 4-1BB ligand (4-1BBL) with similar efficacy[2]. The interaction of 4-1BBL with 4-1BB provides costimulatory signals to both CD4+ and CD8+ T cells through the activation of NF-κB, c-Jun and p38 downstream pathways. 4-1BBL can enhance antitumor responses in the absence of CD28, however for protective immunity, CD28:B7 signaling is necessary[3]. |
