Recombinant Human Parathyroid Hormone, 1-84a.a.是礦物離子代謝的主要調(diào)節(jié)劑。
Synonyms
rHuPTH; Parathormone; Parathyrin
Species
HumanSource
E. coli Accession
P01270 Gene ID
5741 Molecular Weight
Approximately 9,000 Da AA Sequence
SVSEIQLMHN LGKHLNSMER VEWLRKKLQD VHNFVALGAP LAPRDAGSQR PRKKEDNVLV ESHEKSLGEA DKADVNVLTK AKSQ Biological Activity
The ED50 is <0.1 μg/mL as measured by UMR106 cell/cAMP method, corresponding to a specific activity of >1.0 × 104 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 1.15 mg sodium citrate, sodium chloride 7.31 mg, 0.21 mg citric acid, 0.1117 mg EDTA-Na2, 0.2 mg Tween 80 and 50 mg Mannitol. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Parathyroid Hormone, 1-84a.a. (rHuPTH (1-84a.a.)) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Parathyroid Hormone, 1-84a.a. (rHuPTH (1-84a.a.)) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant Human Parathyroid Hormone, 1-84a.a. is a full-length replica of the endogenous Parathyroid hormone molecule, which consists of an amino (N)-terminal structure (first 34 amino acids) and a carboxyl (C)-terminal structure (last 50 amino acids). Full-length recombinant human parathyroid hormone [rhPTH (1-84); Natpara] is approved in the USA as an adjunct to calcium and vitamin D therapy for control of hypocalcaemia in patients with hypoparathyroidism. Parathyroid Hormone regulates calcium and phosphorus levels by acting on the bone, kidneys and intestines. In the bone, Parathyroid hormone mobilizes calcium and phosphorus into the circulation by stimulating bone resorption. Parathyroid hormone stimulates osteoblasts to increase the expression of receptor activator of nuclear factor j-B ligand (RANKL)[1]. |
