Recombinant Human Vascular Endothelial Growth Factor 165 (P. pastoris) 是血管內(nèi)皮生長(zhǎng)因子A (VEGF-A) 的亞型。 VEGF是血管內(nèi)皮細(xì)胞特異性的肝素結(jié)合生長(zhǎng)因子，能夠誘導(dǎo)血管生成。
Synonyms
rHuVEGF165; VPF; Folliculostellate cell-derived growth factor; Glioma-derived endothelial cell mitogen
Species
HumanSource
P. pastoris Accession
P15692 Gene ID
7422 Molecular Weight
Approximately 38.2 kDa AA Sequence
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR Biological Activity
The ED50 is 1-5 ng/mL as measured by HUVEC cells, corresponding to a specific activity of 2 × 105 - 1 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0. Endotoxin Level
<0.5 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Vascular Endothelial Growth Factor 165 (P.pastoris-expressed) (rHuVEGF165) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Human Vascular Endothelial Growth Factor 165 (P.pastoris-expressed) (rHuVEGF165) is stored at -20°C. After reconstitution, it is stable at 4°C for 4 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Vascular Endothelial Growth Factor (VEGF) has multiple isoforms, created by alternative splicing or proteolytic cleavage, and characterized by different receptor-binding and matrix-binding properties. These isoforms are known to give rise to a spectrum of angiogenesis patterns marked by differences in branching, which has functional implications for tissues. VEGF-A is a key member of the VEGF family of cytokines, along with VEGF-B, -C, -D, and PlGF. VEGF-A mediates angiogenesis, the expansion of an existing vascular bed by sprouting of new blood vessels. The vegfa gene is translated into a number of splice isoforms, the most notable in humans being VEGF121, VEGF165, and VEGF189[1]. |
