Recombinant Rat Interleukin-5 是一種 T 細胞衍生的細胞因子，刺激 B 細胞生長并增強免疫球蛋白分泌。
Synonyms
rRtIL-5; EDF; BCDFII; TRF
Species
RatSource
E. coli Accession
Q9R2C9 Gene ID
24497 Molecular Weight
Approximately 26.0 kDa AA Sequence
MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV Biological Activity
The ED50 is <0.4 ng/mL as measured by TF-1 cells, corresponding to a specific activity of >2.5 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 20 mM Tris, pH 8.5. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Interleukin-5 (rRtIL-5) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Rat Interleukin-5 (rRtIL-5) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin-5 (IL-5) is a T-cell derived cytokine which stimulates eosinophil production and activation in human, mice and sheep. IL-5 is active as a growth factor for mouse but not human B cells. The role of IL-5 on ruminant B cells has not been clearly defined. By hybridisation with human IL-5 cDNA, the ovine IL-5 gene is isolated from a liver genomic library. The IL-5 cDNA is obtained by reverse-transcriptase PCR using primers designed from the 5' and 3' coding sequence derived from the ovine IL-5 gene. The sequences of the cDNA shows that there is 79% and 73% nucleotide homology with the human and mouse sequences. The ovine IL-5 cDNA encodes a protein of 132 amino acids and the level of amino acid homology with human and mouse IL-5 is 64% and 56%, respectively. Two cysteine residues are conserved in ovine, human and mouse IL-5. There are two potential N-linked glycoyslation sites in ovine IL-5[1]. |
