Recombinant Rat Interleukin-10 是由多種哺乳動物細(xì)胞類型產(chǎn)生的免疫抑制細(xì)胞因子，包括巨噬細(xì)胞，單核細(xì)胞，B細(xì)胞，T細(xì)胞和角質(zhì)形成細(xì)胞。
Synonyms
rRtIL-10; CSIF
Species
RatSource
E. coli Accession
P29456 Gene ID
25325 Molecular Weight
Approximately 18.7 kDa AA Sequence
MSKGHSIKGD NNCTHFPVSQ THMLRELRAA FSQVKTFFQK KDQLDNIVLT DSLLQDFKGY LGCQALSEMI KFYLVEVMPQ AENHGPEIKE HLNSLGEKLK TLWIQLRRCH RFLPCENKSK AVEQVKNDFN KLQDKGVYKA MNEFDIFINC IEAYVTLKMK N Biological Activity
The ED50 is <0.5 μg/mL as measured by C6 cells, corresponding to a specific activity of >2.0 × 103 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Rat Interleukin-10 (rRtIL-10) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Rat Interleukin-10 (rRtIL-10) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin-10 is an anti-inflammatory Th1 immunosuppressive cytokine, the active form of which is a non-covalent homodimer, and which exhibits species-specificity both with respect to structure and biological activity. The rat homologue of IL-10 shares 73% identity with human IL-10 at the amino-acid sequence level, and has, in addition to the two disulphide bonds present in human IL-10, a fifth, unpaired cysteine (cys-149). Preparation of rat IL-10 by bacterial expression followed by solubilisation and refolding in a glutathione redox system, results in a molecule in which cys-149 is almost entirely oxidised, existing either as disulphide dimer or as a mixed disulphide with glutathione, and which has less than 1% of the activity of the native (cys-149-SH) form of the molecule. Site directed mutagenesis of rat IL-10 to replace cys-149 with tyrosine produces a molecule which readily adopts the active conformation upon solubilisation and refolding, and which is recoverable in good yield from bacterial expression systems. Comparison of the biological activities of rat IL-10tyr149 and commercial rat IL-10 preparations confirms that the activity of native-sequence rat IL-10 is either reduced or absent. It is proposed therefore that the biosynthetic analogue ratIL-10tyr149 is a more useful molecule to investigate the biological actions of IL-10 in the rat[1]. |
