Recombinant Mouse Platelet-derived Growth Factor-BB 是一種有活性的 PDGF 亞型，可用于細(xì)胞培養(yǎng)。
Synonyms
rMuPDGF-BB; PDGF-2; GDGF; ODGF; SIS; SSV ; 重組小鼠血小板衍生生長因子-BB
Species
MouseSource
E. coli Accession
P31240 Gene ID
18591 Molecular Weight
Approximately 24.7 kDa AA Sequence
MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT Biological Activity
The ED50 is <2.5 ng/mL as measured by 3T3 cells, corresponding to a specific activity of >4 × 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 10 mM Sodium Citrate, pH 3.0. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Murine Platelet-derived Growth Factor-BB (rMuPDGF-BB) to 100 μg/mL using ddH2O. Storage & Stability
Lyophilized recombinant Murine Platelet-derived Growth Factor-BB (rMuPDGF-BB) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Platelet-derived growth factor-BB (PDGF-BB) is primarily secreted from platelet α-granules and is the most active PDGF isoform in bone and other connective tissue as it can bind to all known PDGF receptors. PDGF-BB plays an important role in bone regeneration by inducing mitogenesis, chemotaxis, extracellular matrix formation, and vascularization[1]. |
