Recombinant Mouse Interleukin-7 (CHO-expressed) 由來自骨髓和胸腺細胞產生，在 B 細胞淋巴細胞生成和 T 細胞穩(wěn)態(tài)中起關鍵作用。
Synonyms
rMuIL-7; LP-1; pre-B cell factor
Species
MouseSource
CHO Accession
P10168 Gene ID
16196 Molecular Weight
8-28 kDa AA Sequence
ECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSIH HHHHH Biological Activity
The ED50 is <0.4ng/mL as measured by 2E8 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Murine Interleukin-7 (CHO-expressed) (rMuIL-7) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Murine Interleukin-7 (CHO-expressed) (rMuIL-7) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin-7 (IL-7) is a nonredundant cytokine produced by nonlymphoid cells that is essential for T-cell development in humans and mice and B-cell development in mice. IL-7 contributes to the maintenance of the size and subset composition of the peripheral T-cell pool by providing growth and survival signals through the IL-7 receptor. The IL-7 receptor has a private α-chain and shares the common γ-chain (γc) with other common cytokine receptor users, such as IL-2, IL-4, IL-15, and IL-21. IL-7 together with these cytokines regulates the expansion and contraction of adaptive immune responses and potentially the memory response to a given antigen. IL-7 modulates memory CD8+ T cells in response to a virus infection and, given in conjunction with adoptive transfer of T cells after herpes simplex virus type 1 infection, improves virus clearance[1]. |
