Recombinant Mouse Interleukin-2 是一種免疫調(diào)節(jié)細(xì)胞因子，具有廣泛的功能，包括促進(jìn)細(xì)胞凋亡，增殖和淋巴細(xì)胞的存活。
Synonyms
rMuIL-2; IL2; T-cell Growth Factor; TCGF; Aldesleukin
Species
MouseSource
E. coli Accession
P04351 Gene ID
16183 Molecular Weight
Approximately 17.2 kDa AA Sequence
APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ Biological Activity
The ED50 is <0.2 ng/mL as measured by murine CTLL-2 cells, corresponding to a specific activity of >5.0 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Murine Interleukin-2 (rMuIL-2) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1 % BSA. Storage & Stability
Lyophilized recombinant Murine Interleukin-2 (rMuIL-2) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Interleukin-2 (IL-2) is a multi-faceted cytokine, known for promoting proliferation, survival, and cell death depending on the cell type and state. For example, IL-2 facilitates cell death only in activated T cells when antigen and IL-2 are abundant. The availability of IL-2 clearly impacts this process. IL-2 is retained in blood vessels by heparan sulfate, and that biologically active IL-2 is released from vessel tissue by heparanase. The morphologic changes and rapid cell death induced by dimeric IL-2 imply that cell death is mediated by disruption of membrane permeability and subsequent necrosis. IL-2 has a direct and unexpectedly broad influence on cellular homeostatic mechanisms in both immune and non-immune systems[1]. |
