Recombinant Mouse Interleukin-10 (CHO-expressed) 是一種有效的 CHO 細(xì)胞來源的抗炎細(xì)胞因子，作用于感染了神經(jīng)性冠狀病毒的小鼠，增強(qiáng)免疫介導(dǎo)的脫髓鞘作用。
Synonyms
rMuIL-10; CSIF
Species
MouseSource
CHO Accession
P18893 Gene ID
16153 Molecular Weight
21-23 kDa AA Sequence
SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS Biological Activity
The ED50 is <0.2 ng/mL as measured by MC/9 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Murine Interleukin-10 (CHO-expressed) (rMuIL-10) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Murine Interleukin-10 (CHO-expressed) (rMuIL-10) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
The anti-inflammatory cytokine interleukin-10 (IL-10) is a pleiotropic cytokine that is produced in abundant quantities during most parasitic, bacterial, viral, and fungal diseases. IL-10 functions by dampening the innate or very early T cell immune response. Treatment with IL-10 may be useful adjunct therapy in some types of viral encephalitis. IL-10 primarily acts to suppress macrophages and dendritic cell (DC) function by inhibiting expression of major histocompatibility complex (MHC) class II and costimulatory molecules such as CD80/CD86 and production of proinflammatory cytokines and chemokines, including IL-12. IL-10 also has direct effects on T cells, inhibiting activation and cytokine expression. Production of IL-10 by highly activated virus-specific T cells raises the possibility that IL-10 functions via both autocrine and paracrine signaling to limit inflammation during acute phases of the disease[1]. |
