Recombinant Mouse Interleukin-1 beta 是由單核細(xì)胞，組織巨噬細(xì)胞，角質(zhì)形成細(xì)胞和其他上皮細(xì)胞產(chǎn)生的一種促炎細(xì)胞因子。
Synonyms
rMuIL-1β; Catabolin; IL1F2; IL-1 beta; IL1B
Species
MouseSource
E. coli Accession
P10749 Gene ID
16176 Molecular Weight
Approximately 17.5 kDa AA Sequence
MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS Biological Activity
The ED50 is <2.0 pg/mL as measured by mouse D10S cells, corresponding to a specific activity of >5.0 × 108 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Murine Interleukin-1 beta (rMuIL-1β) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Murine Interleukin-1 beta (rMuIL-1β) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
IL-1β is a potent proinflammatory cytokine, whose levels are increased in patients with alcoholic liver disease (ALD). IL-1β is produced as inactive pro–IL-1β (encoded by pro-Il-1b) in response to inflammatory stimuli, including both microbial products and endogenous danger-associated molecules. IL-1β gene expression and synthesis of pro-IL-1β occurs after activation of pattern recognition receptors (PRRs). Inflammatory stimuli also drive activation of cytosolic CARD and PYHIN domain-containing PRRs that recruit ASC and caspase-1 (Casp-1) to assemble into the multiprotein complex inflammasome. Pro-Casp-1 (encoded by pro-Casp-1), activated by the inflammasome, cleaves pro–IL-1β into the bioactive IL-1β. IL-1β acts in an autocrine/paracrine manner via the type I IL-1 receptor (IL-1R1). Activation of IL-1R1 is inhibited by its binding to the IL-1 receptor antagonist (IL-1Ra), a naturally occurring cytokine whose function is to prevent the biologic response to IL-1. Treatment with IL-1Ra significantly improves symptoms in patients with rheumatoid arthritis or autoinflammatory syndromes; unlike anti-TNF therapy, it is not associated with infectious complications[1]. |
