Recombinant Mouse Basic Fibroblast Growth Factor 是一種內(nèi)皮細胞和成纖維細胞的有效有絲分裂原和化學(xué)誘導(dǎo)劑。
Synonyms
rMubFGF; HBGF-2; FGF-2; FGF-b; FGF-basic ; 重組小鼠堿性成纖維細胞生長因子
Species
MouseSource
E. coli Accession
P15655 Gene ID
14173 Molecular Weight
Approximately 16.5 kDa AA Sequence
MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS Biological Activity
The ED50 is <1.0 ng/mL as measured by murine balb/c 3T3 cells, corresponding to a specific activity of >1.0 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Murine Basic Fibroblast Growth Factor (rMubFGF) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1 % BSA. Storage & Stability
Lyophilized recombinant Murine Basic Fibroblast Growth Factor (rMubFGF) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Basic fibroblast growth factor (bFGF) is a heparin-binding mitogen for mesoderm-derived cells, also acts as a mitogen, differentiation inducing and maintenance factor for many neuroectodermal cells including glial cells, neurons, paraneurons, and their tumor counterparts. The molecule is expressed in several types of neuroectodermal cells. bFGF occurs in many neuronal target tissues, and can prevent ontogenetic as well as lesion-induced neuron death[1]. In vitro, basic fibroblast growth factor induces an angiogenic phenotype in endothelial cells, which includes chemotaxis, mitogenesis, protease production, beta-integrin expression, and tube formation in three-dimensional gels. It acts by binding to specific tyrosine kinase receptors and to cell-associated heparan sulfate proteoglycans[2]. |
