Recombinant Human Platelet-derived Growth Factor-BB 是血小板衍生生長(zhǎng)因子 (PDGF) 中最有效的亞型，能夠與 PDGFR 結(jié)合，促進(jìn)不同類型細(xì)胞增殖和骨發(fā)生，進(jìn)一步刺激骨折或缺損的骨生成。
Synonyms
rHuPDGF-BB; PDGF-2; GDGF; ODGF; SIS; SSV ; 重組人血小板衍生生長(zhǎng)因子-BB
Species
HumanSource
E. coli Accession
P01127 Gene ID
5155 Molecular Weight
Approximately 24.8 kDa AA Sequence
MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT Biological Activity
The ED50 is <3 ng/mL as measured by murine Balb/c 3T3 cells, corresponding to a specific activity of >3.3 × 105 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS, pH 7.4. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Platelet-derived Growth Factor-BB (rHuPDGF-BB) to 0.1-1.0 mg/mL using sterile distilled water or aqueous buffer containing 0.1 % BSA. Storage & Stability
Lyophilized recombinant Human Platelet-derived Growth Factor-BB (rHuPDGF-BB) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant Human Platelet-derived Growth Factor-BB is the most active PDGF isoform, binds to PDGF receptor, promotes diverse cell types proliferation and osteogenesis, and further stimulates bone formation in fracture or defect. Platelet-derived growth factor-BB (PDGF-BB) is primarily secreted from platelet α-granules and is the most active PDGF isoform in bone and other connective tissue as it can bind to all known PDGF receptors. PDGF-BB plays an important role in bone regeneration by inducing mitogenesis, chemotaxis, extracellular matrix formation, and vascularization[1]. Recombinant Human Platelet-derived Growth Factor-BB (rhPDGF-BB) accelerates tendon healing by improving matrix remodeling, increased collagen synthesis, and increased cell proliferation. Recombinant Human Platelet-derived Growth Factor-BB is also efficacious in a non-ruptured, degenerated, tendinopathy model. Furthermore, Recombinant Human Platelet-derived Growth Factor-BB addresses chronic tendinopathies by inducing proliferation and migration of progenitor cells and tenocytes, which stimulate structural repair of the degenerated tendon[2]. |
