Recombinant Human Noggin (CHO-expressed) 是骨形態(tài)發(fā)生蛋白 (BMPs) 的拮抗劑，對 hBMP-4 有很高的親和性，而對 hBMP-7 的親和性較低，它在骨骼發(fā)展過程中是必不可少的。
Synonyms
rHuNoggin; NOG ; 重組人頭蛋白 (CHO 細(xì)胞表達(dá))
Species
HumanSource
CHO Accession
Q13253 Gene ID
9241 Molecular Weight
29-31 kDa AA Sequence
QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC Biological Activity
The ED50 is <2.5 ng/mL as measured by ATDC5 cells. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against PBS. Endotoxin Level
<0.2 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Noggin (CHO-expressed) (rHuNoggin) to 100 μg/mL using ddH2O or diluted with PBS. Storage & Stability
Lyophilized recombinant Human Noggin (CHO-expressed) (rHuNoggin) is stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Human Noggin protein has 205 amino acids (residues 28-232) after removal of its signal sequence (residues 1-27) and is secreted as a glycosylated, covalently linked homodimer, binds with high affinity to hBMP-4, and with lower affinity to hBMP-7 and acts as an antagonist of bone morphogenetic proteins (BMPs)[1]. Noggin is essential for proper skeletal development; excess BMP activity in the Noggin null mutant results in excess cartilage and failure to initiate joint formation[2]. |
