Recombinant Human Macrophage Colony-stimulating Factor 是一種造血生長因子，具有多個糖基化位點，能夠影響巨噬細胞的生存和功能，同時具有抗腫瘤活性。
Synonyms
rHuM-CSF; CSF-1; MGI-IM ; 重組人巨噬細胞群刺激因子
Species
HumanSource
E. coli Accession
P09603 Gene ID
1435 Molecular Weight
Approximately 28 kDa AA Sequence
MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS Biological Activity
The ED50 is 1 - 3 ng/mL as measured by M-NFS-60 cells, corresponding to a specific activity of 3.3 × 105 - 1 × 106 units/mg. Appearance
Lyophilized powder. Formulation
Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0. Endotoxin Level
<1 EU/μg, determined by LAL method. Reconstitution
Reconstitute the lyophilized recombinant Human Macrophage Colony-stimulating Factor (rHuM-CSF) to 100 μg/mL using ddH2O or PBS or Tris-HCl, pH 8.0. Storage & Stability
Lyophilized recombinant Human Macrophage Colony-stimulating Factor (rHuM-CSF) is stored at -20°C. After reconstitution, it is stable at 4°C for 2 weeks or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage. Shipping
Room temperature in continental US; may vary elsewhere. Background
Recombinant Human Macrophage Colony-stimulating Factor is a hematopoietic growth factor with various glycosylation sites, affects survival and function of the tissue macrophages, and possesses antitumor activity[1]. Macrophage Colony Stimulating Factor (M-CSF) is a pro-inflammatory cytokine, constitutively produced by several cell types, such as fibroblasts, endothelial cells, stromal cells, macrophages, smooth muscle cells and osteoblasts, binds to its receptor CSF1R, and exists in several isoforms- as a secreted glycoprotein, a cell-surface protein and a proteoglycan[2]. M-CSF is involved in the development and proliferation of cells of the monocyte/macrophage lineage and participates in the induction of osteoclasts, which are important in the destruction of bone and cartilage and in the periarticular osteoporotic changes seen in patients with rheumatoid arthritis[3] |
